Research/art/teacher profile of a person
Name and surname:
doc. RNDr. Stanislav Stuchlík, CSc.
Document type:
Research/art/teacher profile of a person
The name of the university:
Comenius University Bratislava
The seat of the university:
Šafárikovo námestie 6, 818 06 Bratislava

I. - Basic information

I.1 - Surname
Stuchlík
I.2 - Name
Stanislav
I.3 - Degrees
doc. RNDr., CSc.
I.4 - Year of birth
1964
I.5 - Name of the workplace
Department of Molecular Biology, Faculty of Natural Sciences Comenius Univ in Bratislava
I.6 - Address of the workplace
Mlynská dolina, Ilkovičova 6, 842 15 Bratislava
I.7 - Position
associate professor
I.8 - E-mail address
stanislav.stuchlik@uniba.sk
I.9 - Hyperlink to the entry of a person in the Register of university staff
https://www.portalvs.sk/regzam/detail/4465
I.10 - Name of the study field in which a person works at the university
Molecular biology and Biotechnology
I.11 - ORCID iD
https://orcid.org/0000-0001-9510-4061

II. - Higher education and further qualification growth

II.1 - First degree of higher education
II.2 - Second degree of higher education
II.a - Name of the university or institution
Faculty of Natural Sciences in Bratislava
II.b - Year
1987
II.c - Study field and programme
Biochemistry
II.3 - Third degree of higher education
II.a - Name of the university or institution
Faculty of Natural Sciences in Bratislava
II.b - Year
1995
II.c - Study field and programme
Biochemistry
II.4 - Associate professor
II.a - Name of the university or institution
Faculty of Natural Sciences in Bratislava
II.b - Year
2007
II.c - Study field and programme
Molecular biology
II.5 - Professor
II.6 - Doctor of Science (DrSc.)

III. - Current and previous employment

III.a - Occupation-position III.b - Institution III.c - Duration
research associate Dept. Biochem, FNS CU 1987 - 1992
research associate Dept. Mol. Biol., FNS CU 1992 - 1995
researcher - lecturer Dept. Mol. Biol., FNS CU 1995 - 2008
associate professor Dept. Mol. Biol., FNS CU 2008 -
head of dept. Dept. Mol. Biol., FNS CU 2019 -

IV. - Development of pedagogical, professional, language, digital and other skills

IV.a - Activity description, course name, other IV.b - Name of the institution IV.c - Year
„IPR Protection at the Higher Education”, Bucurest, RO WIPO Geneva 2007

V. - Overview of activities within the teaching career at the university

V.1 - Overview of the profile courses taught in the current academic year according to study programmes
V.1.a - Name of the profile course V.1.b - Study programme V.1.c - Degree V.1.d - Field of study
Methods in Molecular Biology Biology and Medical Biology I. Biology
Regulation of gene expression in prokaryots Biology and Medical Biology I. Biology
Intellectual property protection in Biotech Molecular Biology and Biotechnology II. Biotechnology
Molecular Immunology Molecular Biology and Biotechnology II. Biology
Selected topics in Molecular Biology Molecular Biology and Biotechnology II. Biology
V.2 - Overview of the responsibility for the delivery, development and quality assurance of the study programme or its part at the university in the current academic year
V.2.a - Name of the study programme V.2.b - Degree V.2.c - Field of study
Molecular biology II. Biology
Molecular biology III. Biology
Biotechnology II. Biotechnology
Biotechnology III. Biotechnology
V.3 - Overview of the responsibility for the development and quality of the field of habilitation procedure and inaugural procedure in the current academic year
V.3.a - Name of the field of habilitation procedure and inaugural procedure V.3.b - Study field to which it is assigned
Molecular biology Biology
V.4 - Overview of supervised final theses
V.4.1 - Number of currently supervised theses
V.4.a - Bachelor's (first degree)
3
V.4.b - Diploma (second degree)
4
V.4.c - Dissertation (third degree)
3
V.4.2 - Number of defended theses
V.4.a - Bachelor's (first degree)
45
V.4.b - Diploma (second degree)
52
V.4.c - Dissertation (third degree)
14
V.5 - Overview of other courses taught in the current academic year according to study programmes
V.5.a - Name of the course V.5.b - Study programme V.5.c - Degree V.5.d - Field of study
Seminar in MB1, Seminar in MB2 Biology and Medical Biology I. Biology
Laboratory topics and calculation in MB Biology and Medical Biology I. Biology
Special methods ina DNA analysis Biology and Medical Biology I. Biology
Legislation and biosafety in biotech Molecular Biology and Biotechnology II. Biology
Advances in Mol Biol 1 Molecular Biology and Biotechnology II. Biology

VI. - Overview of the research/artistic/other outputs

VI.1 - Overview of the research/artistic/other outputs and the corresponding citations
VI.1.1 - Number of the research/artistic/other outputs
VI.1.a - Overall
415
VI.1.b - Over the last six years
202
VI.1.2 - Number of the research/artistic/other outputs registered in the Web of Science or Scopus databases
VI.1.a - Overall
93 WoS
VI.1.b - Over the last six years
28 WoS
VI.1.3 - Number of citations corresponding to the research/artistic/other outputs
VI.1.a - Overall
295
VI.1.b - Over the last six years
83
VI.1.4 - Number of citations registered in the Web of Science or Scopus databases
VI.1.a - Overall
494
VI.1.b - Over the last six years
230
VI.1.5 - Number of invited lectures at the international, national level
VI.1.a - Overall
10
VI.1.b - Over the last six years
5
VI.2 - The most significant research/artistic/other outputs
1

Vávrová, S., Struhárňanská, E., Turňa, J., Stuchlík, S. The tellurium, a rare element with influence on prokaryotic and eukaryotic biological systems. Int. J. Mol. Sci. 2021, 22, 5924. - Q1

2

Varga, V., Štefuca, V., Mihálová, L., Levarski, Z., Struhárňanská, E., Blaško, J., Kubinec, R., Farkaš, P., Sitkey, V., Turňa, J., Rosenberg, M., Stuchlík, S. Recombinant enzymatic redox systems for preparation of aroma compounds by biotransformation. Frontiers in Microbiology 12: 684640, 2021 -   Q1

3

Kormanová; Ľ., Rybecká; S., Levarski; L., Struhárňanská; E., Levarská; L., Blaško; J., Turňa; J., Stuchlík, S. Comparison of simple expression procedures in novel expression host Vibrio natriegens and established Escherichia coli system. Journal of Biotechnology 321: 57-67, 2020 - Q1

4

Stuchlík, S., Turna, J. Current status of biotechnology in Slovakia. Current Opinion in Biotechnology 24 S1, S14-18, 2013 - Q1

5

Burian, J., Stuchlík, S., Kay, W.W: Replication Control of a Small Cryptic Plasmid of Escherichia coli. J. Mol. Biol. 294, 49-66, 1999,  IF1999 = 5,5

VI.3 - The most significant research/artistic/other outputs over the last six years
1

Vávrová, S., Struhárňanská, E., Turňa, J., Stuchlík, S. The tellurium, a rare element with influence on prokaryotic and eukaryotic biological systems. Int. J. Mol. Sci. 2021, 22, 5924. - Q1

2

Varga, V., Štefuca, V., Mihálová, L., Levarski, Z., Struhárňanská, E., Blaško, J., Kubinec, R., Farkaš, P., Sitkey, V., Turňa, J., Rosenberg, M., Stuchlík, S. Recombinant enzymatic redox systems for preparation of aroma compounds by biotransformation. Frontiers in Microbiology 12: 684640, 2021 - Q1

3

Kormanová; Ľ., Rybecká; S., Levarski; L., Struhárňanská; E., Levarská; L., Blaško; J., Turňa; J., Stuchlík, S. Comparison of simple expression procedures in novel expression host Vibrio natriegens and established Escherichia coli system. Journal of Biotechnology 321: 57-67, 2020 - Q1

4

Minich A., Levarski Z., Mikulášová M., Straka, M., Liptáková, A.,, Stuchlík, S. Complex analysis of vanillin and syringic acid as natural antimicrobial agents against Staphylococcus epidermidis biofilms. Int. J. Mol. Sci. 23, art. No. 1816, 2022. - Q1

5

Kormanová, Ľ., Levarski, Z., Minich, A., Varga, V., Levarská, L., Struhárňanská, E., Turňa, J., Stuchlík, S. Novel efficient recombinant expression system based on enhanced permeability of Vibrio natriegens induced by D,D-carboxypeptidase overexpression. World Journal of Microbiology and Biotechnology 39, Art. No. 277, 2023 - Q2

VI.4 - The most significant citations corresponding to the research/artistic/other outputs
1

Burian, J., Stuchlík, S., Kay, W.W: Replication Control of a Small Cryptic Plasmid of Escherichia coli. J. Mol. Biol. 294, 49-66, 1999

2000 Solar, G., Espinosa, M. Plasmid copy number control: an ever-growing story. Molecular Microbiology 37: (3) 492-500,2000

2006 Srivastava P et al. Characterization of broad host range cryptic plasmid pCR1 from Corynebacterium renale. Plasmid 56(1): 24-34,2006                     

2011 Fratamico, P.M. etal. The complete DNA sequence and analysis of the virulence plasmid and of five additional plasmids carried by Shiga toxin-producing Escherichia coli O26:H11 strain H30. International Journal of Medical Microbiology 301 (3),192-203,2011 01

2012 Ma, YF. et al. Analysis of two marine metagenomes reveals the diversity of plasmids in oceanic environments. Environmental Microbiology 14 (2), 453-466,2012

2019 Branger, C. et al. Specialization of small non-conjugative plasmids in Escherichia coli according to their family types. Microbial Genomics 5 (9), Article Number: 000281, 2019

2023 Dewan, I., Uecker, H. A mathematician’s guide to plasmids: an introduction to plasmid biology for modellers. Microbiology (United Kingdom) 169(7), Art. No. 001362, 2023

2

Gajdošová, J., Benedikovičová, K., Kamodyová, N., Tóthová, Ľ., Kaclíková, E., Stuchlík, S., Turňa, J., Drahovská, H. Analysis of the DNA region mediating increased thermotolerance at 58°C in Cronobacter sp. and other enterobacterial strains. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 100, 279-289,2011

2012 Joseph, S. et al. Comparative Analysis of Genome Sequences Covering the Seven Cronobacter Species. PLoS ONE 7(11): Art. No. e49455, 2012            

2013 Grim, C.J. et al. Pan-genome analysis of the emerging foodborne pathogen Cronobacter spp. suggests a species-level bidirectional divergence driven by niche adaptation. BMC Genomics 14, Art. No. 366,2013

2015 The H.C. et al. A high-resolution genomic analysis of multidrug resistant hospital outbreaks of Klebsiella pneumoniae. EMBO Molecular Medicine 7: 227-239,2015

2020 Li, H. et al. Heat and Pressure Resistance in Escherichia coli Relates to Protein Folding and Aggregation. Frontiers in Microbiology 11, Art. No 111,2020

2021 Almblad, H. et al. Bacterial cyclic diguanylate signaling networks sense temperature. Nature Communications 12, Art. No. 1986, 2021

2024 Liu, H., et al Diverse Genotypes of Cronobacter spp. Associated with Dairy Farm Systems in Jiangsu and Shandong Provinces in China. Foods 13(6):871, 2024

2024 Soni, A., Brightwell, G. Genetic determinants of thermal resistance in foodborne bacterial pathogens. Food Safety and Health 2: 213–220, 2024

3

Levarski Z., Šoltýsová A., Krahulec, J., Stuchlík, S., Turňa, J. High-level expression and purification of recombinant human growth hormone produced in soluble form in Escherichia coli. Protein Expression and Purification 100, 40-47, 2014

2018 Dai, Z. et al. Genetic tool development and systemic regulation in biosynthetic technology. Biotechnology for Biofuels 11: 152,2018

2018 Jang S.H. et al. Development of bicistronic expression system for the enhanced and reliable production of recombinant proteins in Leuconostoc citreum. Scientific Reports 8 (1), Art. No. 8852,2018

2020 Perez‐Perez, D.A. et al. Engineered small metal‐binding protein tag improves the production of recombinant human growth hormone in the periplasm of Escherichia coli. FEBS Open Bio 10: 546–551,2020

2021 Amaranto, M. et al. Novel intein-based self-cleaving affinity tag for recombinant protein production in Escherichia coli., Journal of Biotechnology 332: 126-134,2021

2021 Keshavarz, R. et al. Over-expression, Overproduction, Purification and characterization of rhGH in E. coli. Biotechnology and Applied Biochemistry 68: 122–135, 2021

2023 Wang, Y. et al. Growth hormone receptor agonists and antagonists: From protein expression and purification to long‐acting formulations. Protein Science 32(9): e4727, 2023

2024 Nasr, S.M. et al. Interdisciplinary gene manipulation, molecular cloning, and recombinant expression of modified human growth hormone isoform-1 in E. coli system. International Journal of Biological Macromolecules 257, 128637, 2024

4

Madyagol, M., Al-Alami, H., Levarski, Z., Drahovská, H., Turňa, J., Stuchlík, S. Gene replacement techniques for Escherichia coli genome modification. Folia Microbiologica 56, 253-263, 2011

2015 Liu W. et al. Generation of an attenuated strain oral vaccine candidate using. Appl Microbiol Biotechnol 99: 855–867, 2015,                                     

2016 Liu, L. et al. L-tryptophan production in Escherichia coli improved by weakening the Pta-AckA pathway. PLoS ONE 11 (6), Art.No. e0158200, 2016      

2019 Chen, TP. et al. Efficient Biofilm-Based Fermentation Strategies for L-Threonine Production by Escherichia coli. Frontiers in Microbiology 10, Art. No.: 1773, 2019

2020 Xu, X. et al. Extension of Genetic Marker List Using Unnatural Amino Acid System: An Efficient Genomic Modification Strategy in Escherichia coli. Frontiers in Bioengineering and Biotechnology 8, Art. No 145, 2020

2021 Saleski, T.E. et al. Optimized gene expression from bacterial chromosome by high-throughput integration and screening. Science Advances 7 (7): Art. No.eabe1767, 2021

2022 Liu, QG. et al. Type I fimbriae subunit fimA enhances Escherichia coli biofilm formation but affects L-threonine carbon distribution. Frontiers in Bioengineering and Biotechnology 10, Art. No- 904636, 2022

2024 Kamali, F. et al. Chapter 5-Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli. In: Progresses in Ammonia: Science, Technology and Membranes (Ed.A. Basile and M.R. Rahimpou), pp. 113-142, 2024, Elsevier

5

Vávrová, S., Struhárňanská, E., Turňa, J., Stuchlík, S. The tellurium, a rare element with influence on prokaryotic and eukaryotic biological systems. Int. J. Mol. Sci. 22(11), 5924; 2021

2023 Liu, W. et al. Biosynthesis of uniform fluorescent-stable telluride quantum dots in Escherichia coli and its detection of Fe3+ in water. Biochemical and Biophysical Research Communications 667: 153-161, 2023

2023 O’Loughlin, E.J. et al. Tellurium Goes for a Ride on the “Ferrous” Wheel: Interactions of Te(VI) and Te(IV) with Fe(II)-Bearing Minerals. ACS Earth and Space Chemistry 7 (10): 1825-1836, 2023

2024 Sári, D., et al. Tellurium and Nano-Tellurium: Medicine or Poison? Nanomaterials 14(8):670, 2024

2024 Kińska, K. et al. Interaction of Te(IV) and Te(VI) with the soil matrix – Sorption and fractionation as a function of soil composition. Environmental Pollution 361,124878, 2024

2024 Tozawa, M. et al. Spectrally Narrow Blue-Light Emission from Nonstoichiometric AgGaS2 Quantum Dots for Application to Light-Emitting Diodes. ACS Applied Materials & Interfaces, 16(49): 68169-68180, 2024   

VI.5 - Participation in conducting (leading) the most important research projects or art projects over the last six years
1

APVV-14-0375 (2015 - 2019, applied research) - Synthetic biology and production of peroxidases de novo - deputy of principal investigator

2

APVV-17-0333 (2018 - 2022, applied research) - Research and development od effective processes of vanillin and other natural aromes production with using oxidative and protective effect of recombinant catalase and peroxidase - principal investigator

3

APVV-21-0215 (2022-2026, applied research) - Bacterial Lon protease as an emerging tool for. multiple myeloma treatment - principal investigator

4

APVV-19-0196 (2020 - 2024) - Neuroendocrine regulation of energetic metabolism in model Drosophila melanogaster - deputy of principal investigator

5

APVV-22-0161 (2023-2027, applied research) - The use of biotechnology transformation in the production healthy beverages - deputy of principal investigator

European Space Agency (Noordwijk, NL) GRANT_NUMBER: Sixth Call for Outline Proposals under the Plan for European Cooperating States (PECS) in Slovakia/ESA, The European Space Research and Technology Centre (ESTEC) (2022-2024) - Mapping of Arthrospira PCC8005 lipidom - deputy of principal investigator

Horizon Europe program under grant agreement No. 101160008 - Forgenome II project (2024 - 2027) - member of

VII. - Overview of organizational experience related to higher education and research/artistic/other activities

VII.a - Activity, position VII.b - Name of the institution, board VII.c - Duration
Member of experts board Comission for biol. safety at Min. of Env. SR from 01/2020
Member ENGL working group on „Detection of genetically modified microorganisms in food and feed" European Commission, Directorate-general, Joint Research Centre, Brussels 11/2020 -
Member of Council and Secretary Slovak Society for Bioch. and Mol. Biol. - FEBS member from 02/2019
Editorial Board member Journal Amino Acids (IF=3,5) from 05/2017
Special Issue Editor Journal Biomolecules (IF=4,5) from 04/2021

VIII. - Overview of international mobilities and visits oriented on education and research/artistic/other activities in the given field of study

VIII.a - Name of the institution VIII.b - Address of the institution VIII.c - Duration (indicate the duration of stay) VIII.d - Mobility scheme, employment contract, other (describe)
Dept. Mol. Genetics, Leiden University, The Netherlands Leiden, The Netherlands 06/1994-07/1994 TEMPUS program - short stay
Dept. Bioch. and Microbiol., University of Victoria, B.C., Canada Victoria, B.C., Canada 1995-1997 NSERC Ottawa - NATO Science Postdoctoral Fellowship Award
Dept. Mol. Genetics, Leiden University, The Netherlands Leiden, The Netherlands 2000-2002 Postdoctoral fellowship, EC project: Structural studies on the mechanism of DNA excision repair

IX. - Other relevant facts

IX.a - If relevant, other activities related to higher education or research/artistic/other activities are mentioned

Vicedean of FNS CU for development and IT - 04/2009 –01/2011                     

External expert-delegate of MinEnv SR at COP MOP 8 - 8. Conference of UN agreement on Biol. Safety, Cartagena protokol, 0212.-19.12.2016, Cancún, Mexico                          

Thank you letter from the Dean of FNS CU on the occasion of the 75th anniversary of the establishment of the faculty in 2015 for research results - member of the awarded team of the Department of Molecular Biology                 

Cooperation WIPO Geneva: „WIPO Initiative University IPR coordinator“ at FNS CU (2003-2010)        

Cooperation with private sector:

1. CPN Dolní Dobrouč, ČR/Czech Rep.: 2007-2011research contact - cooperation with production of hyaluronic acid, production of antimicrobial peptides; supervising PhD. student in extermal form study)

2. Axxence Slovakia/Monoprix, s.r.o.: long-term cooperation (from 2007 - 2022) on research of aromatic additives biotransformation by recombinant enzymes in the frame of APVV projects.

3. hameln rds, Modra (formerly VUL Modra) in 1992-1994 - contract research, in 2010-2014 - common ERDF research project "Industrial research of new therapeuticals based on recombinant proteins".

4. Medirex, a.s., Bratislava: long-term cooperation with organizing Medirex Group Academy award for best diploma thesis in molecular biology and immunology with clinical practical use, and supervising of student mol. biol. in the frame Bc. and MSc. theses in Medirex; coordination with preparation of strategic research cooperation.

1 granted patent - Industrial property rights office of SR:                                                                           

1. SK patent, No. 289111 (UPV SR, 2023) - Stuchlík, S., Levarski, Z., Struhárňanská, E., Zámocký M, Turňa. J. Polypeptide of recombinant catalase-peroxidase, the procedure for its production in Escherichia coli cells and its use. PP50015-2019, 15.3.2019, ÚPV SR

Patent applications - Industrial property rights office of SR                                                                                                                       

1. Bocánová L., Krahulec, J., Stuchlík, S., Turňa, J. Procedure for construction of host strain Escherichia coli with dual regulation of expression of recombinant proteins. PP 5039-2013, 17.12.2013, ÚPV SR

2. Krahulec, J., Levarski, Z. Stuchlík, S., Turňa, J. Procedure for production of soluble human recombinant growth hormone. PP5038-2014, 17.12.2013, ÚPV SR

3. Stuchlík, S., Levarski, Z., Koiš, P., Fraňo, M., Turňa. J. Procedure of trans-2-hexenal to trans-2-hexenol biotransformation by recombinant enzymes. PP50068-2016. 13.10.2016, ÚPV SR

4. Stuchlík, S. Levarski, Z. Kormanová, Ľ., Turňa, J. The method of permeability increase of outer cells structures in Vibrio natriegens from point of view of recombinant protein transport. PP 50063-2021, 06.12.2021, ÚPV SR

Date of last update
2025-03-22