Title: Study of the protective effect of Monophosphoryl lipid A on UVC-irradiated THP-1 monocytes Author (s): Monika Dobrotková, Martina Djobeková, Martina Hrčka Dubničková Source: European Pharmaceutical Journal [elektronický dokument]. - Roč. 70, č. S2 (2023), s. 22-22 [online] https://sciendo.com/article/10.2478/afpuc-2023-002Published: 2024

Title: Praktické cvičenia z mikrobiológie pre farmaceutov Author (s): Drobná, Eva, Hrčka Dubničková, Martina, Greifová, Gabriela [elektronický dokument]. - 1. vyd. - Bratislava : Farmaceutická fakulta UK, 2021. - 174 s. [online] ISBN 978-80-223-5123-2

Title: Imunologické metódy [elektronický dokument] : princípy a návody na praktické cvičenia. Author(s): Kiňová Sepová, Hana, Bilková, Andrea, Hrčka Dubničková, Martina Dudík, Boris - 1. vyd. - Bratislava : Univerzita Komenského v Bratislave, 2021. - 146 s. [online] ISBN 978-80-223-5124-9

Title: Modifying Effect of Different Forms of Lipid A on the Induction of DNA Double-Strand Breaks in Mice Hippocampal Cells After Exposure to Ionizing Radiation of Different Quality in Vitro Author (s): Kuzmina, EA; Chausov, VN; Dubnickova M; Gaevsky, VN; Ilyina, EV; Kozhina, RA; Timoshenko, GN; Tiounchik, SI; Boreyko, AV Source: American Institute of Physics conference proceedings of the 23rd International Scientific Conference of Young Scientists and Specialists Volume: 2163 Article Number: 050002 DOI: 10.1063/1.5130106 ISBN: 978-0-7354-1908-7 Published: 2019

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Pisárčik, Martin - Dubničková, Martina - Devínsky, Ferdinand - Lacko, Ivan - Škvarla, J. : Dynamic Light Scattering and Electrokinetic Potential of Bis(Quarternary Ammonium Bromide) Surfactant Micelles as the Function of the Alkyl Chain Length. In: Colloids and Surfaces A - Physicochemical and Engineering Aspects. - Vol. 143, No. 1, 1998, s. 69-75.

1. [n1] 2022 Shaban, SM, Hamed, EH, Elsharif, AM, Elged, AH, El Basiony, NM, F Shaban, Samy M., Hamed, Eman H., Elsharif, Asma M., Elged, Ahmed H., El Basiony, N. M.: Preparation gemini non-ionic surfactants-based polyethylene oxide with variable hydrophobic tails for controlling the catalytic and   antimicrobial activity of AgNPs, JOURNAL OF MOLECULAR LIQUIDS, 0167-7322, 1873-3166, 2022, 367, SCI
2. [n1] 2021 Ahmadova, GA, Rahimov, RA, Abilova, Z, Huseynova, KA, Imanov, E, Zubkov, FI: Effect of head-group of cationic surfactants and structure of ionic groups of anionic polyelectrolyte in oppositely charged  polymer-surfactant complexes, SO COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 0927-7757, 1873-4359, 2021, 613, SCI
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VEGA 2/0429/21 Štúdium mechanizmov modulácie zápalu a lipidového metabolizmu laktobacilmi v modeli nealkoholovej tukovej choroby pečene - spoluriešiteľ

Projekt je zameraný na štúdium mechanizmov účinku 4 kmeňov laktobacilov (i) na zápalový proces a lipidový metabolizmus v modeli pečene (HepG2) a (ii) na porušenú črevnú bariéru (Caco-2) v in vitro podmienkach. V prvej etape sa zameriame na štúdium TLR4 signálnej dráhy a jej „downstream“ molekúl, na aktiváciu tvorby protizápalových cytokínov a na stimuláciu expresie negatívnych regulátorov TLR4 signálnej dráhy v pečeni. Budeme sledovať vplyv laktobacilov na reguláciu lipidového metabolizmu (influx, efflux, syntéza a degradácia cholesterolu, triacylgycerolov, mastných kyselín). V druhej etape optimalizujeme zápalový kokultivačný model enterocytov (Caco-2) s hepatocytmi (HepG2). Na molekulárnej úrovni budeme analyzovať schopnosť laktobacilov obnoviť porušenú permeabilitu monovrstvy Caco-2 a ovplyvniť zápalový proces a lipidový metabolizmus v modeli medziorgánovej komunikácie v čase. Našimi poznatkami by sme chceli prispieť k objasneniu mechanizmov vzniku NAFLD a možnostiam jej ovplyvnenia probiotikami

VEGA 1/0394/18 Fosfatidylinozitolový systém a transkripčné faktory v kontexte sekundárnych metabolitov-zástupca vedúceho projektu

Projekt bol zameraný na štúdium fosfatidylinozitol-4-fosfát-5-kinázy (PI4P-5K, EC 2.7.1.67) a transkripčných faktorov WRKY/MYB v kontexte signálnych systémov a sekundárneho metabolizmu rastlín. Ako experimentálny model sa použije mak siaty (Papaver somniferum L.). Vplyv fosfolipidovej signalizácie na produkciu a akumuláciu sekundárnych metabolitov rastlín nebol doteraz dôkladne preskúmaný. Na mieste je teda otázka: Môže mať fosfatidylinozitolový systém vplyv na biosyntézu benzylizochinolínových alkaloidov? A aké funkcie majú pri týchto procesoch transkripčné faktory? Cieľom projektu je identifikácia kompletnej cDNA sekvencie kódujúcej makovú PI4P-5K, sledovanie transkripčného levelu WRKY/MYB, stanovenie profilu alkaloidov (morfín, kodeín, tebaín, papaverín) a génových transkriptov pre enzýmy biosyntézy alkaloidov (TYDC, CNMT, 6-OMT, 4-OMT, SalAT, COR).

KEGA 064UK-4/2018 Inovácia náplne praktických cvičení z predmetov Imunológia a Mikrobiológia a vypracovanie účebných textov - spoluriešiteľ

Cieľom projektu bolo vypracovať aktualizované vysokoškolské učebné texty pre praktické cvičenia z povinných predmetov Imunológia a Mikrobiológia pre študentov študijného programu Farmácia, ktoré zohľadnia novodobé poznatky a zmeny vo výučbe spomínaných predmetov s ohľadom na farmaceutický, resp. medicínsky aspekt. Súčasťou projektu bol aj výber a zavedenie moderných metodík, vhodných pre realizáciu v podmienkach vysokoškolských laboratórií. Aktualizácia a príprava učebných textov sa netýkala len ich obsahu, ale aj formy. Konkrétnym výstupom sú dva nové učebné texty: Imunologické metódy: princípy a návody na praktické cvičenia autorov Kiňová Sepová H., Bilková A., Hrčka Dubničková M., Dudík B. (FaF UK, Bratislava 2021) a Praktické cvičenia z mikrobiológie pre farmaceutov autorov Drobná E., Hrčka Dubničková M., Greifová G. (FaF UK, Bratislava 2021), ktoré sú prístupné všetkým študentom Farmaceutickej fakulty UK v on line forme. Okrem návodov oboje skriptá obsahujú aj teoretické úvody k jednotlivým úlohám. Nájdu uplatnenie v praktickej časti výučby daných predmetov, ale majú ambíciu byť aj materiálom vhodným pre samoštúdium a prípravu na skúšky z týchto predmetov.

LRB SÚJV Dubna, Ruská federácia s podtémou Biologická odpoveď na nízke dávky radiácie, ktoré sú súčasťou riešenej témy č. 04-9-1077-04-9-1077-2015/2023: Výskum biologického pôsobenia ťažkých nabitých častíc s rôznou energiou - vedúca projektu zo strany SR/prof. Krasavin zo strany SUJV RF - ukončené k jún 2022

V spolupráci s Rádiobiologickým laboratóriom SÚJV Dubna sa riešila téma Research of Biological Action of heavy Charged Particles with Different Energy č. 04-9-1077-2015/23 (podtéma Biological responses to low dose radiation). Ďalej sa sledoval vplyv gama žiarenia na apoptózu a nekrózu imunokompetentných buniek po pôsobení uvedených lipidov A. V uvedených bunkách sa pokračovalo v sledovaní poškodenia DNA po pôsobení gama žiarenia a regenerácii buniek difosforyl- a monofosforyllipidu A in vivo. Akalickou verziou gélovej elektroforézy - kometového testu (Comet assay) sa in vitro zistila detekcia jedno- a dvojreťazcových zlomov DNA na úrovni leukocytov. Z predchádzajúcich výsledkov in vitro vyplynulo, že najväčší počet zlomov v DNA bol pozorovaný pri vzorkách samotného ožiarenia, ktorý koreloval s bunkami regenerovanými difosforyllipidom A a znížený počet zlomov bol pozorovaný vo vzorkách leukocytov po pôsobení monofosforyllipidu A. Pokračovať v experimentoch na ochranu nervových buniek hippokampusu po pôsobení gama žiarenia (60Co) sa plánuje aj ďalej, výsledky sa vzájomne porovnajú a bude popísaná hypotéza ochrannej schopnosti MLA na jednotlivé typy buniek z hľadiska štatisticky významného výsledku daného zámeru. Celý zámer je uskutočnený zo zámeru ochrany buniek imunitného systému a hippokampusu z limbického systému mozgu človeka, ktorý hrá dôležitú úlohu pri konsolidácii informácií z krátkodobej pamäti do dlhodobej pamäti a hlavne priestorovej orientácie v podmienkach ionizačného žiarenia.