Research/art/teacher profile of a person
Name and surname:
PharmDr. Katarína Maráková, PhD.
Document type:
Research/art/teacher profile of a person
The name of the university:
Comenius University Bratislava
The seat of the university:
Šafárikovo námestie 6, 818 06 Bratislava

I. - Basic information

I.1 - Surname
Maráková
I.2 - Name
Katarína
I.3 - Degrees
PharmDr., PhD.
I.4 - Year of birth
1984
I.5 - Name of the workplace
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University Bratislava
I.6 - Address of the workplace
Odbojárov 10, 832 32 Bratislava
I.7 - Position
Associate Professor
I.8 - E-mail address
marakova@fpharm.uniba.sk
I.9 - Hyperlink to the entry of a person in the Register of university staff
www.portalvs.sk/regzam/detail/5274
I.10 - Name of the study field in which a person works at the university
Pharmacy
I.11 - ORCID iD
0000-0003-2170-6582

II. - Higher education and further qualification growth

II.1 - First degree of higher education
II.2 - Second degree of higher education
II.a - Name of the university or institution
Faculty of Pharmacy, Comenius University Bratislava
II.b - Year
2007
II.c - Study field and programme
Pharmacy
II.3 - Third degree of higher education
II.a - Name of the university or institution
Faculty of Pharmacy, Comenius University Bratislava
II.b - Year
2011
II.c - Study field and programme
Pharmaceutical Chemistry
II.4 - Associate professor
II.5 - Professor
II.6 - Doctor of Science (DrSc.)

III. - Current and previous employment

III.a - Occupation-position III.b - Institution III.c - Duration
Assistant Professor Faculty of Pharmacy, Comenius University Bratislava 2011-2022
pharmacist Pharmacy Na korze 2008-2009
pharmacist Pharmacy U dvoch levov 2007-2008
Associate Professor Faculty of Pharmacy, Comenius University Bratislava 2022 - to date

IV. - Development of pedagogical, professional, language, digital and other skills

IV.a - Activity description, course name, other IV.b - Name of the institution IV.c - Year
Development of LC-MS methods for small molecules School of Mass Spectrometry (Czech Spectroscopic Society) 2019
Statistical Data Evaluation School of Mass Spectrometry (Czech Spectroscopic Society) 2019, 2022
Basics of proteomics School of Mass Spectrometry (Czech Spectroscopic Society) 2023
Biologics by Mass Spectrometry School of Mass Spectrometry (Czech Spectroscopic Society) 2023

V. - Overview of activities within the teaching career at the university

V.1 - Overview of the profile courses taught in the current academic year according to study programmes
V.1.a - Name of the profile course V.1.b - Study programme V.1.c - Degree V.1.d - Field of study
Analytical Chemistry Pharmaceutical chemistry III. Pharmacy
Chemical diagnostic and medical devices, their properties and standardization Medical and Diagnostic devices I. Medical Sciences
V.2 - Overview of the responsibility for the delivery, development and quality assurance of the study programme or its part at the university in the current academic year
V.2.a - Name of the study programme V.2.b - Degree V.2.c - Field of study
Medical and Diagnostic devices I. Medical Sciences
V.3 - Overview of the responsibility for the development and quality of the field of habilitation procedure and inaugural procedure in the current academic year
V.4 - Overview of supervised final theses
V.4.1 - Number of currently supervised theses
V.4.b - Diploma (second degree)
4
V.4.c - Dissertation (third degree)
2
V.4.2 - Number of defended theses
V.4.b - Diploma (second degree)
32
V.5 - Overview of other courses taught in the current academic year according to study programmes
V.5.a - Name of the course V.5.b - Study programme V.5.c - Degree V.5.d - Field of study
Analysis of Substances in Biological Systems Pharmacy II. Pharmacy
New Trends in Analytical Chemistry Pharmacy II. Pharmacy
Analytical Chemistry 1 Pharmacy II. Pharmacy
Analytical Chemistry 2 Pharmacy II. Pharmacy

VI. - Overview of the research/artistic/other outputs

VI.1 - Overview of the research/artistic/other outputs and the corresponding citations
VI.1.1 - Number of the research/artistic/other outputs
VI.1.a - Overall
158
VI.1.b - Over the last six years
51
VI.1.2 - Number of the research/artistic/other outputs registered in the Web of Science or Scopus databases
VI.1.a - Overall
38
VI.1.b - Over the last six years
11
VI.1.3 - Number of citations corresponding to the research/artistic/other outputs
VI.1.a - Overall
480
VI.1.b - Over the last six years
220
VI.1.4 - Number of citations registered in the Web of Science or Scopus databases
VI.1.a - Overall
479
VI.1.b - Over the last six years
220
VI.1.5 - Number of invited lectures at the international, national level
VI.1.a - Overall
3
VI.1.b - Over the last six years
3
VI.2 - The most significant research/artistic/other outputs
1

ADC: K. Maráková, A.J. Rai, K.A. Schug, Effect of difluoroacetic acid and biological matrices on the development of a liquid chromatography-triple quadrupole mass spectrometry method for determinationof intact growth factor proteins, Journal of Separation Science. Vol. 43, No. 9-10 (2020), p. 1663-1677. (invited paper to Special Issue: Emerging Thought Leaders in Separation Science)

2

V3: K. Maráková, M. Opetová, R. Tomašovský., K.A. Schug, Greenness of proteomic sample preparation and analysis techniques

for biopharmaceuticals. Trends in Analytical Chemistry, Vol. 171 (2024), art. no. 117490, (invited paper to Special Issue "Greenness Metrics for Analytical Methodologies")

3

ADC: K. Maráková, J. Piešťanský, Z. Zelinková, P. Mikuš. Simultaneous determination of twelve biogenic amines in human urine as potential biomarkers of inflammatory bowel diseases by capillary electrophoresis - tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. Vol. 186 (2020), p. [1-12], art. no. 113294 

4

ADC: P. Mikuš, K. Maráková, J. Marák, J. Nemec, I. Valášková, E. Havránek. Direct quantitative determinationof amlodipine enantiomers in urine samples for pharmacokinetic study using on-line coupled isotachophoresis-capillary zone electrophoresis separation method with diode array detection. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences. Vol. 875, No. 1 (2008), p. 266-272. 

5

V3: K. Maráková, B.J. Renner, S.L.Thomas, M. Opetová, R. Tomašovský, A.J. Rai, K.A. Schug. Solid phase extraction as sample pretreatment method for top-down quantitative analysis of low molecular weight proteins from biological samples using liquid chromatography – triple quadrupole mass spectrometry. Analytica Chimica Acta Vol. 1243 (2023), art. no. 340801.

VI.3 - The most significant research/artistic/other outputs over the last six years
1

ADC: K. Maráková, A.J. Rai, K.A. Schug, Effect of difluoroacetic acid and biological matrices on the development of a liquid chromatography-triple quadrupole mass spectrometry method for determinationof intact growth factor proteins, Journal of Separation Science. Vol. 43, No. 9-10 (2020), p. 1663-1677. (invited paper to Special Issue Emerging Thought Leaders in Separation Science)

2

V3: K. Maráková, M. Opetová, R. Tomašovský., K.A. Schug, Greenness of proteomic sample preparation and analysis techniques

for biopharmaceuticals. Trends in Analytical Chemistry, Vol. 171 (2024), 117490, (invited paper to Special Issue "Greenness Metrics for Analytical Methodologies")

3

ADC: S.L. Thomas, J.B. Thacker, K.A. Schug, K. Maráková, Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis. Journal of Separation Science Vol. 44, No. 1 (2021), p. 211-246. (invited paper to Special Issue Reviews 2021)

4

ADC: K. Maráková, J. Piešťanský, Z. Zelinková, P. Mikuš. Simultaneous determination of twelve biogenic amines in human urine as potential biomarkers of inflammatory bowel diseases by capillary electrophoresis - tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. Vol. 186 (2020), p. [1-12], art. no. 113294 

5

V3: K. Maráková, B.J. Renner, S.L.Thomas, M. Opetová, R. Tomašovský, A.J. Rai, K.A. Schug. Solid phase extraction as sample pretreatment method for top-down quantitative analysis of low molecular weight proteins from biological samples using liquid chromatography – triple quadrupole mass spectrometry. Analytica Chimica Acta, Vol. 1243 (2023), art. no. 340801.

VI.4 - The most significant citations corresponding to the research/artistic/other outputs
1

Rocco, A. - Donati, E. - Touloupakis, E. - Aturki, Z.: TrAC - Trends in Analytical Chemistry, Vol. 103, 2018, s. 183 

2

Neususs, C. - Romer, J. - Hocker, O. - Jooss, K.: Coupling of capillary electromigration techniques to mass spectrometry. In: Capillary Electromigration Separation Methods. [s.l.] : Elsevier, 2018, S. 311 

3

Takemori, A. - Kaulich, P. T. - Cassidy, L. - Takemori, N. - Tholey, A.: Analytical Chemistry, Vol. 94, No. 37, 2022, s.12815 - 12821 

4

Luo, Y. - Wang, B. - Yi, L. - Ding, C.F. - Deng, C. - Yan, Y.: Mesoporous materials for glycopeptide separation. In: TrAC - Trends in Analytical Chemistry, Vol. 167, 2023, art. no. 117234

5

Kaulich, P.T. - Cassidy, L. - Winkels, K. - Tholey, A.: Improved Identification of Proteoforms in Top-Down Proteomics Using FAIMS with Internal CV Stepping. In: Analytical Chemistry, Vol. 94, No. 8, 2022, s. 3600-3607  

VI.5 - Participation in conducting (leading) the most important research projects or art projects over the last six years
1

VEGA 1/0483/20 “Novel Approaches in Pretreatment of Biological Samples for Analysis of Intact Proteins as Disease Biomarkers”

  • principal investigator
  • Variable protein expression in biological fluids can correlate with the progression of numerous diseases, including cancer. Thus, reliable analysis of proteins in biological samples is important, since these molecules can serve as potential disease biomarkers. Trace intact protein biomarker analysis in complex biological samples is still a very challenging research area, where a successful analytical procedure requires use of an efficient sample preparation prior to high efficiency separation and high specificity detection. Sample preparation is a necessary, crucial and often the most time-consuming part in a proteomic workflow. In the current project, we aim to develop simple, reliable and innovative sample preparation approaches based on extraction, coacervation, as well as more advanced preparative isotachophoresis. These studies will fill a gap in providing proper rapid solutions for targeted analysis of intact proteins as disease biomarkers in biological samples
2

VEGA 1/0463/18 “New approaches in analytical evaluation of drugs, their biodegradation products and metabolic markers in biological matrices related to optimization of therapy of inflammatory bowel diseases”

  • co-investigator


3

APVV-15-0585 „Analytical Evaluation of Clinical Sample Profiles and Markers Related to Optimization Thiopurines Therapy of Nonspecific Bowel Disease“

  • co-investigator


4

VEGA 1/0514/22 „Advanced analytical monitoring of thiopurine nucleotides and anti-drug antibodies in combined biological therapy of inflammatory bowel diseases“

  • co-investigator
5

Fellowships for excellent researchers R2-R4 09I03-03-V04-00192, "Greening Sample Preparation and Analysis in Proteomics"

  • principal investigator
  • Anotation: Proteins are biomolecules with many physiological functions, which also participate in many pathological processes in the human body. Therefore, these molecules currently represent one of the most effective drugs and are also attractive targets in the search for new potential biomarkers. From an analytical point of view, the characterization and quantification of proteins, due to their very complex structure, still represents a great challenge. Especially when it comes to the analysis of proteins in intact form and/or complex biological matrices. The climate crisis and the need to reduce the impact of human activity on the environment have been a very important topic for the last few decades, even within analytical chemistry. Thus, it is necessary to focus on the development of greener analytical methods. In addition to the search for greener solvents for traditionally used liquid chromatography, capillary electrophoresis is increasingly being applied in the analysis of biomolecules. In addition to the separation step itself, effective sample pretreatment is essential for proteomic analyses in complex samples. Therefore, the development of new more ecological pretreatment and analytical procedures for the analysis of proteins in complex biological samples is a very current and important analytical field. The development of such analytical workflows within the presented project can become a valuable basis for further application outputs

VII. - Overview of organizational experience related to higher education and research/artistic/other activities

VII.a - Activity, position VII.b - Name of the institution, board VII.c - Duration
member of organization committee of international conference 46th EuroCongress on Drug Synthesis and Analysis 09/2016 – 09/2017
member of scientific committee EuSP2024 and GSAC2024, Chania, Greece 2024

VIII. - Overview of international mobilities and visits oriented on education and research/artistic/other activities in the given field of study

VIII.a - Name of the institution VIII.b - Address of the institution VIII.c - Duration (indicate the duration of stay) VIII.d - Mobility scheme, employment contract, other (describe)
University of Texas at Arlington Arlington, Texas, USA 10/2018 - 03/2019 National Scholarship Programme of the Slovak Republic
University of Texas at Arlington Arlington, Texas, USA 01/2021 - 07/2021 Fulbright scholarship
Palacky University in Olomouc Olomouc, Czech Republic 05/2024 (1 wee) Erasmus+

IX. - Other relevant facts

IX.a - If relevant, other activities related to higher education or research/artistic/other activities are mentioned

2017   Scientific Degree IIa (Slovak Academy of Science)


2017, 2018    Slovak semifinal of the international program “For Women in Science”


2021 - present    EuChemS-DAC Sample Preparation Study Group and Network (member)

2019 - present    American Society for Mass Spectrometry (ASMS) (member)

2022 - present Czech Association of Mass Spectrometry (member)

2023 - present Slovak Chemical Society (member)

2023 - present co-editor (European Pharmaceutical Journal)

2024 - present co-editor (Journal of Separation Science)

2024 - present member of advisory board (Separation Science Plus)

Date of last update
2025-02-19