Research/art/teacher profile of a person
Name and surname:
prof. RNDr. Iveta Waczulíková, PhD.
Document type:
Research/art/teacher profile of a person
The name of the university:
Comenius University Bratislava
The seat of the university:
Šafárikovo námestie 6, 818 06 Bratislava

I. - Basic information

I.1 - Surname
Waczulíková
I.2 - Name
Iveta
I.3 - Degrees
Prof. RNDr., PhD.
I.4 - Year of birth
1966
I.5 - Name of the workplace
Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University
I.6 - Address of the workplace
Mlynská dolina, 84248 Bratislava
I.7 - Position
Professor
I.8 - E-mail address
waczulikova@fmph.uniba.sk
I.9 - Hyperlink to the entry of a person in the Register of university staff
https://www.portalvs.sk/regzam/detail/4780
I.10 - Name of the study field in which a person works at the university
Physics
I.11 - ORCID iD
0000-0002-1749-2780

II. - Higher education and further qualification growth

II.1 - First degree of higher education
II.2 - Second degree of higher education
II.a - Name of the university or institution
Comenius University in Bratislava
II.b - Year
1990
II.c - Study field and programme
Biophysics
II.3 - Third degree of higher education
II.a - Name of the university or institution
Comenius University in Bratislava
II.b - Year
2000
II.c - Study field and programme
Biochemistry
II.4 - Associate professor
II.a - Name of the university or institution
Comenius University in Bratislava
II.b - Year
2010
II.c - Study field and programme
Physics
II.5 - Professor
II.a - Name of the university or institution
Comenius University in Bratislava
II.b - Year
2024
II.c - Study field and programme
Physics
II.6 - Doctor of Science (DrSc.)

III. - Current and previous employment

III.a - Occupation-position III.b - Institution III.c - Duration
Professor Comenius University in Bratislava, Faculty of Mathematics, Physics and Informatics 2024-
Associate Professor (Dozent) Comenius University in Bratislava, Faculty of Mathematics, Physics and Informatics 2010-2024
assistant lecturer / researcher Comenius University in Bratislava, Faculty of Mathematics, Physics and Informatics 2000-2010
assistant lecturer / researcher Comenius University in Bratislava, Medical Faculty 1996

IV. - Development of pedagogical, professional, language, digital and other skills

IV.a - Activity description, course name, other IV.b - Name of the institution IV.c - Year
TBL101 Course (Team-Based Learning) Advanced TBL Course Comenius University in Bratislava, Faculty of Medicine (Certified by New York University (NYU) School of Medicine) 2018
Certificate in Biomedical Statistics I, II and III Statsoft (Dell Software) CR, Prague, CZ 2009-2010
Cambridge ESOL (English for Speakers of Other Languages) CAE - Certificate in Advanced English University of Cambridge, absolvované na: St. Mark’s International College, Perth, Western Australia 2003-2004

V. - Overview of activities within the teaching career at the university

V.1 - Overview of the profile courses taught in the current academic year according to study programmes
V.1.a - Name of the profile course V.1.b - Study programme V.1.c - Degree V.1.d - Field of study
Introduction to biostatistics Biomedical physics I. Physics (main field), General Medicine (secondary field)
Bachelor thesis project Biomedical physics I. Physics (main field), General Medicine (secondary field)
Bachelor thesis seminar Biomedical physics I. Physics (main field), General Medicine (secondary field)
Experimental methods of medical physics (1) Biomedical physics II. Physics (main field), General Medicine (secondary field)
Methodology of preparation and implementation of research projects Biophysics III. Physics
V.2 - Overview of the responsibility for the delivery, development and quality assurance of the study programme or its part at the university in the current academic year
V.2.a - Name of the study programme V.2.b - Degree V.2.c - Field of study
Biomedical Physics I. and II. Physics (master) and General Medicine (secondary)
Biophysics III. Physics
V.3 - Overview of the responsibility for the development and quality of the field of habilitation procedure and inaugural procedure in the current academic year
V.4 - Overview of supervised final theses
V.4.1 - Number of currently supervised theses
V.4.a - Bachelor's (first degree)
2
V.4.b - Diploma (second degree)
0
V.4.c - Dissertation (third degree)
3
V.4.2 - Number of defended theses
V.4.a - Bachelor's (first degree)
23
V.4.b - Diploma (second degree)
45
V.4.c - Dissertation (third degree)
6
V.5 - Overview of other courses taught in the current academic year according to study programmes
V.5.a - Name of the course V.5.b - Study programme V.5.c - Degree V.5.d - Field of study
Medical devices Biomedical physics I. Physics (main field), General Medicine (secondary field)
Introduction to biostatistics and probability Medical Biology I. Biology
Design of Experiments and Studies in Biomedical Research Biomedical physics II. Physics (main field), General Medicine (secondary field)
Specifics of Interdisciplinary Teamwork Biomedical physics II. Physics (main field), General Medicine (secondary field)
Methods for Preparation and Implementation of Research Projects Biophysics III. Physics

VI. - Overview of the research/artistic/other outputs

VI.1 - Overview of the research/artistic/other outputs and the corresponding citations
VI.1.1 - Number of the research/artistic/other outputs
VI.1.a - Overall
719
VI.1.b - Over the last six years
176
VI.1.2 - Number of the research/artistic/other outputs registered in the Web of Science or Scopus databases
VI.1.a - Overall
181
VI.1.b - Over the last six years
58
VI.1.3 - Number of citations corresponding to the research/artistic/other outputs
VI.1.a - Overall
1617
VI.1.b - Over the last six years
858
VI.1.4 - Number of citations registered in the Web of Science or Scopus databases
VI.1.a - Overall
1617
VI.1.b - Over the last six years
848
VI.1.5 - Number of invited lectures at the international, national level
VI.1.a - Overall
27
VI.1.b - Over the last six years
12
VI.2 - The most significant research/artistic/other outputs
1

Andelová, Natália, Waczulíková, Iveta,; Kunstek, Lukáš, Talian, Ivan, Ravingerová, Táňa, Jašová, Magdaléna, Šutý, Šimon, Ferko, Miroslav: Dichloroacetate as a metabolic modulator of heart mitochondrial proteome under conditions of reduced oxygen utilization. Scientific Reports [e-document]. - 12, No. 1 (2022), s. [1-16], art. no. 16348 [online]London : ISSN 2045-2322

2

Profant, M., Sláviková, K., Kabátová, Z., Slezák, P., & Waczulíková, I. (2012). Predictive validity of MRI in detecting and following cholesteatoma. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 269(3), 757–765. https://doi.org/10.1007/s00405-011-1706-8

3
Waczulíková, Iveta, Šikurová, Libuša. Alterations in membrane structure and dynamics of blood elements and cardiomyocytes in diabetes. In: Advances in Molecular Mechanisms and Pharmacology of Diabetic Complications. - Kerala : Transworld Research Network, 2010. - S. 109-128 [2,086 AH]. - ISBN 978-81-7895-499-8
4

Waczulíková, Iveta, Slezák, Peter: Introductory biostatistics. - 1. vyd. - Bratislava : Comenius University, 2015. - 147 s. ISBN 978-80-223-3938-4

5

Trebatická, J., Kopasová, S., Hradecná, Z., Cinovský, K., Skodácek, I., Suba, J., Muchová, J., Zitnanová, I., Waczulíková, I., Rohdewald, P., & Duracková, Z. (2006). Treatment of ADHD with French maritime pine bark extract, Pycnogenol. European child & adolescent psychiatry, 15(6), 329–335. https://doi.org/10.1007/s00787-006-0538-3

VI.3 - The most significant research/artistic/other outputs over the last six years
1

Bališ P, Berényiová A, Mišák A, Grman M., Rošťáková Z, Waczulíková I, Čačányiová S, Domínguez-Álvarez E, Ondriaš Karol. The Phthalic Selenoanhydride Decreases Rat Blood Pressure and Tension of Isolated Mesenteric, Femoral and Renal Arteries. Molecules, 2023, 28, 4826 (s. 1-18). DOI 10.3390/molecules28124826

2

Michlewska S, Garaiova Z, Šubjakova V, Hołota M, Kubczak M, Grodzicka M, Okła E, Naziris N, Balcerzak Ł, Ortega P, de la Mata FJ, Hianik T, Waczulikova I, Bryszewska M, Ionov M. Lipid-coated ruthenium dendrimer conjugated with doxorubicin in anti-cancer drug delivery: Introducing protocols. Colloids Surf B Biointerfaces. 2023 Jul;227:113371. doi: 10.1016/j.colsurfb.2023.113371. Epub 2023 May 24. PMID: 37244201.

3

Velísková, M., Zvarík, M., Šutý, Š., Jacko, J., Mydla, P., Čechová, K., Džubinská, D., Morvová, M. ml., Ionov, M., Terehova, M.M., Majoral, J.P., Bryszewska, M., Waczulíková, I. In Vitro Interactions of Amphiphilic Phosphorous Dendrons with Liposomes and Exosomes-Implications for Blood Viscosity Changes. In: Pharmaceutics [elektronický dokument]. - Roč. 14, č. 8 (2022), s. 1-17, art. no. 1596 [online]. - ISSN (online) 1999-4923, URL: https://www.mdpi.com/1999-4923/14/8/1596/htm Registrované v: scopus, wos, IF (JCR) 2020=6.321

4

Suty, S.; Oravczova, V.; Garaiova, Z.; Subjakova, V.; Ionov, M.; Shcharbin, D.; Simonikova, Z.; Bartek, P.; Zvarik, M.; Shi, X.; Mignani, S.; Majoral, J.-P.; Bryszewska, M.; Hianik, T.; Waczulikova, I. Blood Compatibility of Amphiphilic Phosphorous Dendrons—Prospective Drug Nanocarriers. Biomedicines 2021, 9, 1672. https://doi.org/10.3390/biomedicines9111672

5

Katrenčíková, B.; Vaváková, M.; Waczulíková, I.; Oravec, S.; Garaiova, I.; Nagyová, Z.; Hlaváčová, N.; Ďuračková, Z.; Trebatická, J. Lipid Profile, Lipoprotein Subfractions, and Fluidity of Membranes in Children and Adolescents with Depressive Disorder: Effect of Omega-3 Fatty Acids in a Double-Blind Randomized Controlled Study. Biomolecules 2020, 10, 1427. https://doi.org/10.3390/biom10101427

VI.4 - The most significant citations corresponding to the research/artistic/other outputs
1
Vanovcanova, L., Lehotska, V., Machalekova, K., Waczulikova, I., Minarikova, E., Rauova, K., & Kajo, K. (2019). Idiopathic Granulomatous Mastitis - a new approach in diagnostics and treatment. Neoplasma, 66(4), 661–668. https://doi.org/10.4149/neo_2019_190201N100 [o1] 2020 Bell, S. - Villasmil, R. - Lattanzio, N. - Abdelal, Q. - King, A. - Farhangi, V.: Journal of Investigative Medicine High Impact Case Reports, vol. 8, 2020, DOI: 10.1177/2324709620949325 - SCI ; SCOPUS [o1] 2021 Yilmaz, M. A. - Kaya, T. I. - Demirel, M. - Yuyucu Karabulut, Y.: Dermatologic Therapy, vol. 34, no. 1, 2021, art. no. e14729 - SCI ; SCOPUS [o1] 2021 Alsaleh, N.: Assertive clinical practice in managing patients with idiopathic granulomatous mastitis: Review of literature. In: Annals of Medicine and Surgery, Vol. 70, 2021, art. no. 102792 - SCOPUS [o1] 2021 Toprak, N. - Gündüz, A. M.: Idiopathic granulomatous mastitis and other types of mastitis; comparison of ultrasound findings. In: Eastern Journal of Medicine, Vol. 26, No. 2, 2021, s. 339-343 - SCOPUS [o1] 2021 Chan, Ch. W.: The treatment conundrum that is idiopathic granulomatous mastitis. In: Annals Academy of Medicine Singapore, Vol. 50, No. 8, 2021, s. 396-397 - SCI
2
Ambrózy, E., Waczulíková, I., Willfort, A., Böhler, K., Cauza, K., Ehringer, H., Heinz, G., Koppensteiner, R., Marić, S., & Gschwandtner, M. E. (2013). Healing process of venous ulcers: the role of microcirculation. International wound journal, 10(1), 57–64. https://doi.org/10.1111/j.1742-481X.2012.00943.x [o1] 2014 Allen, J. - Howell, K.: Microvascular imaging: techniques and opportunities for clinical physiological measurements. In: Physiological Measurement, Vol. 35, No. 7, 2014, s. R91-R141 - SCI ; SCOPUS [o1] 2017 Raposio, E. - Bertozzi, N. - Moretti, R. - Grignaffini, E. - Grieco, M. P.: Laser Doppler Flowmetry and Transcutaneous Oximetry in Chronic Skin Ulcers: A Comparative Evaluation. In: Wounds-A Compendium of Clinical Research andPractice, Vol. 29, No. 7, 2017, s. 190-195 - SCI ; SCOPUS [o1] 2017 Williams, K. J. - Sounderajah, V. - Dharmarajah, B. - Thapar, A. - Davies, A. H.: Simulated Wound Assessment Using Digital Planimetry versus Three-Dimensional Cameras: Implications for Clinical Assessment. In: Annals of VascularSurgery, Vol. 41, 2017, s. 235-240 - SCI ; SCOPUS [o1] 2018 Carow, J. B. - Carow, J. - Gueorguiev, B. - Klos, K. - Herren, C. - Pishnamaz, M. - Weber, C. D. - Nebelung, S. - Kim, B. S. - Knobe, M.: Soft tissue micro-circulation in the healthy hindfoot: a cross-sectional study with focus onlateral surgical approaches to the calcaneus. In: International Orthopaedics, Vol. 42, No. 11, 2018, s. 2705-2713 - SCI ; SCOPUS [o1] 2021 Balasubramanian, G. V. - Chockalingam, N. - Naemi, R.: The Role of Cutaneous Microcirculatory Responses in Tissue Injury, Inflammation and Repair at the Foot in Diabetes. In: Frontiers in Bioengineering and Biotechnology, Vol. 9,2021, art. no. 732753 - SCI ; SCOPUS
3
Trebatická, J., Kopasová, S., Hradecná, Z., Cinovský, K., Skodácek, I., Suba, J., Muchová, J., Zitnanová, I., Waczulíková, I., Rohdewald, P., & Duracková, Z. (2006). Treatment of ADHD with French maritime pine bark extract, Pycnogenol. European child & adolescent psychiatry, 15(6), 329–335. https://doi.org/10.1007/s00787-006-0538-3 [o1] 2007 Weber, W. - Newmark, S.: Pediatric Clinics of North America, vol. 54, no. 6, 2007, s. 983-1006 - SCI ; SCOPUS [o1] 2008 Brown, R. - Gerbarg, P. - Muskin, P. - Roose, S.: Complementary and Alternative Treatments in Psychiatry. In: Psychiatry: Third Edition, Vol. 2, 2008, s. 2318-2353 - SCOPUS [o1] 2015 Selek, S. - Ceylan, M.F.: A relationship between oxidative ... In: Studies on Psychiatric Disorders. Book Series: Oxidative Stress in Applied Basic Research and Clinical Practice. New York : Springer, 2015, s. 143-150 - BKCI-S [o1] 2018 Sawni, A. - Kemper, K.J.: Attention Deficit Disorder. In: Integrative Medicine: Fourth Edition. Amsterdam : Elsevier, 2018, s. 53-63.e4 - SCOPUS [o1] 2021 Fawns, T.: Primary Care, vol. 48, no. 3, spec. iss. Sl, 2021, s. 475-491 - SCI ; SCOPUS
4
Watala, C., Waczulikova, I., Wieclawska, B., Rozalski, M., Gresner, P., Gwoździński, K., Mateasik, A., & Sikurova, L. (2002). Merocyanine 540 as a fluorescent probe of altered membrane phospholipid asymmetry in activated whole blood platelets. Cytometry, 49(3), 119–133. https://doi.org/10.1002/cyto.10152 [o3] 2003 Shapiro, H. M.: Practical Flow Cytometry. Hoboken : Wiley and Sons, 2003, S. 629 [o1] 2004 Gylys, K. H. - Fein, J. A. - Yang, F. H. - Cole, G. M.: Merocyanine 540 as a fluorescent probe of altered membrane phospholipid asymmetryin activated whole blood platelets. In: Cytometry Part A, Vol. 60A, 2004, No. 1, s. 90-96 - SCI; SCOPUS [o1] 2004 Lapotko, D.: Monitoring of amoptosis in intact single cells with photothermal microscope. In: Cytometry Part A, Vol. 58A, 2004, No. 2, s. 111-119 - SCI ; SCOPUS [o1] 2006 Badlou, B. A. - Wu, Y. P. - Smid, W. M. - Akkerman, J. W. N.: Platelet binding and phagocytosis by macroxphages. In: Transfusion, Vol. 46, No. 8, 2006, s. 1432-1443 - SCI ; SCOPUS [o1] 2008 McCarthy, D. A. - Macey, M. G. - Allen, P. D.: A simple, novel, procedure for monitoring membrane scrambling and permeability in microparticles, platelets, and lekocytes in whole blood samples. In: Experimental Hematology, Vol. 36,No. 8, 2008, s. 909-921 - SCI ; SCOPUS [o1] 2019 Kubrak, T. - Podgórski, R. - Aebisher, D. - Galiniak, S. - Bartusik-Aebisher, D.: Experimental uses of flow cytometry. In: Introduction to Flow Cytometry. New York : Nova Science Publishers, 2019, S. 89-119 - SCOPUS
5

Waczulíková, I., Sikurová, L., Bryszewska, M., Rekawiecka, K., Cársky, J., & Ulicná, O. (2000). Impaired erythrocyte transmembrane potential in diabetes mellitus and its possible improvement by resorcylidene aminoguanidine. Bioelectrochemistry (Amsterdam, Netherlands), 52(2), 251–256. https://doi.org/10.1016/s0302-4598(00)00107-0 [o1] 2007 Thiruvengadam, A.P. - Chandrasekaran, K.: Evaluating the validity of blood-based membrane potential changes for the identification of bipolar disorder I. In: Journal of Affective Disorders, Vol. 100, No. 1-3, 2007, s. 75-82 - SCI ;SCOPUS [o1] 2007 Wei, N. - Mi, M.T. - Zhou, Y.: Influences of Lovastatin on membrane ion flow and intracellular signaling in breast cancer cells. In: Cellular & Molecular Biiology Letters, Vol. 12, No. 1, 2007, s. 1-15 - SCI ; SCOPUS [o1] 2011 Kaczmarska, M. - Kopyscianska, Z. - Fornal, M. - Grodzicki, T. - Matlak, K. - Korecki, J. - Burda, K.: Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes. In: Acta Biochimica Polonica, Vol. 58,No. 4, 2011, s. 489-496 - SCI ; SCOPUS [o1] 2011 Labieniec-Watala, M. - Siewiera, K. - Gierszewski, S. - Watala, C.: Mitochondria Function in Diabetes From Health to Pathology New Perspectives for Treatment of Diabetes Driven Disorders. In: Biomedical Science, Engineering andTechnology. Rijeka : InTech, 2011, S. 123-150 - BKCI-S [o1] 2015 Sabnis, R,W.: 3,3 '-dipropylthiadicarbocyanine iodide (DiSC(3)(5)). In: Handbook of Fluorescent Dyes and Probes. Hoboken : John Wiley & Sons Inc., 2015, S. 189-192 - BKCI-S

VI.5 - Participation in conducting (leading) the most important research projects or art projects over the last six years
1

VEGA 1/0634/25: Non-specific physical interactions of nanoparticles investigated for medical use - in vitro effect on hemocoagulation, and viscoelastic and flow properties of blood. 2023-2027 (principal investigator)

2

APVV-22-0154: Cardiovascular protection mediated by α1AMPK against metabolic syndrome-mediated Endothelial Dysfunction – identifying new risk factors. 2023-2027 (team leader, CU)

3

APVV-22-0264: Bioenergetic and proteomic diagnostics in cardioprotection: an effective tool for monitoring the regulation of mitochondrial signaling pathways. 2023-2027. researcher.

4

NAWA EUROPARTNER PPI/APM/2018/1/00007/U/001, Strengthening and spreading international partnership activities of the Faculty of Biology and Environmental Protection for interdisciplinary research and innovation of the University of Lodz. 2019-2022 (team leader of Slovak team)

5

APVV SK-BY-RD-19-0019 (research grant): Analysis of Interaction between Amphiphilic Dendrons and Biological Systems. 2019-2022 (principal investigator)

6

KEGA 041UK-4/2020 Training of a Clinical and Biomedical Physicist - implementation of new biomedical technologies and methods of active learning. 2020-2022 (principal investigator)

VII. - Overview of organizational experience related to higher education and research/artistic/other activities

VII.a - Activity, position VII.b - Name of the institution, board VII.c - Duration
Director of the Bachelor's degree and co-guarantor of the Master's degree study programme in Biomedical physics Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava 2014 - up to now
Co-guarantor of the doctoral study programme in Biophysics Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava 2014 - up to now
Member of the Field Committee for Doctoral Studies of Biophysics Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava 2014 - up to now
Member of the Ethics Board of the Faculty of Mathematics, Physics and Informatics Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava 2021 -
Member of the Field Committee for Doctoral Studies of Biophysics The Faculty of Science of the PJ Šafárik University in Košice 2025

VIII. - Overview of international mobilities and visits oriented on education and research/artistic/other activities in the given field of study

VIII.a - Name of the institution VIII.b - Address of the institution VIII.c - Duration (indicate the duration of stay) VIII.d - Mobility scheme, employment contract, other (describe)
the Institute of Biophysics, University of Lodz, PL (Uniwersytet Łódzki, Wydział Biologii i Ochrony Środowiska, Instytut Biofizyki, Katedra Biofizyki Ogólnej) ul. Pomorska 141/143, 90-236, Łódż, Rector: Narutowicza 65, 90-131 Łódź 1996 (1997-1999 MD) 2000-doteraz mobility v rámci dlhodobej výskumno-vzdelávacej spolupráce, výmenné pobyty viacerých študentov, diplomové a PhD práce / mobilities within a long-term research and educational cooperation, exchange stays of several students working on their diploma and PhD theses
Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz t.č. Narutowicza 96, 90-141 Lodz, Poland 1997 (1997-1999 MD) 2000-2010 mobilities within a research and educational cooperation, exchange stays of several students working on their diploma and PhD theses
Biophysics Unit, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitat Autònoma de Barcelona. 08193 Bellaterra. Catalunya. Spain 08193 Bellaterra, Catalunya, Spain 2006 a mobility with a PhD student, seminars, training, research during the stay within the NATO SECURITY THROUGH SCIENCE PROGRAM, COLLABORATIVE LINKAGE GRANT "Prion proteins and Alzheimer's Peptides: Molecular Studies."
Institute of Biophysics of the Czech Academy of Sciences Královopolská 135, 612 65 Brno 1999- mobility with doctoral students, seminars, consultations

IX. - Other relevant facts

IX.a - If relevant, other activities related to higher education or research/artistic/other activities are mentioned

Member of the Scientific Board of The Centre of Experimental Medicine (CEM) of the Slovak Academy of Sciences (SAS),

Member of the Scientific Board of the Centre of Biosciences SAS,

External statistical advisor to Non-clinical and Clinical Assessment Department at the State Institute for Drug Control, Slovakia,

Activities in the project PREVEDA, focused on promoting young scientists at all stages of their research and education (Editor and section moderator positions from the beginning in 2009) https://www.preveda.sk/informacie

Lecturer in the non-profit organization Premedix Academy, which organizes training courses for doctors and researchers (2020 - 2021)

Editor Open Chemistry, (CC, Scopus) General Physiology and Biophysics (CC, Scopus)

Professional Society Memberships EBSA - European Biophysical Societies' Association SKBS - Slovenská biofyzikálna spoločnosť (Slovak Biophysical Society) ISCB – International Society for Clinical Biostatistics SSDS - Slovak Statistical and Demographical Society – vice-president of SSDS for medical statistics ISHR – International Society for Heart Research, SSBMB - Slovak Society for Biochemistry and Molecular Biology

Date of last update
2025-05-29